Subretinal injection in mice to study retinal physiology and disease

Peirong Huang, Siddharth Narendran, Felipe Pereira, Shinichi Fukuda, Yosuke Nagasaka, Ivana Apicella, Praveen Yerramothu, Kenneth M. Marion, Xiaoyu Cai, Srinivas R. Sadda, Bradley D. Gelfand, Jayakrishna Ambati

Published: 2022-04-12 DOI: 10.1038/s41596-022-00689-4

Subretinal injection

Retinal physiology

Standardized protocol

Mice model

Proficiency training

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Subretinal injection (SRI) is a widely used technique in retinal research and can be used to deliver nucleic acids, small molecules, macromolecules, viruses, cells or biomaterials such as nanobeads. Here we describe how to undertake SRI of mice. This prot
Introduction
Materials
Procedure
Troubleshooting
Timing
Anticipated results
- References
References
ACKNOWLEDGMENTS

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Subretinal injection in mice to study retinal physiology and disease

Extended

Extended Data Fig. 1 Custom needle parameters.

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Supplementary Information

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